agena massarray methylation analysis Search Results


massarray epityper dna methylation technology  (agena bioscience)
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agena bioscience massarray epityper dna methylation technology
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massarray analyzer 4 0  (agena bioscience)
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massarray-system-1  (agena bioscience)
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massarray system  (Sequenom)
90
Sequenom massarray system
( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by <t>MassARRAY</t> shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).
Massarray System, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sample integrity  (agena bioscience)
96
agena bioscience sample integrity
( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by <t>MassARRAY</t> shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).
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sample integrity - by Bioz Stars, 2026-05
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pharmacogenetics  (agena bioscience)
96
agena bioscience pharmacogenetics
( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by <t>MassARRAY</t> shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).
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hereditary genetics  (agena bioscience)
96
agena bioscience hereditary genetics
( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by <t>MassARRAY</t> shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).
Hereditary Genetics, supplied by agena bioscience, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mutation profiling  (agena bioscience)
94
agena bioscience mutation profiling
( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by <t>MassARRAY</t> shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).
Mutation Profiling, supplied by agena bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray compact system  (Sequenom)
90
Sequenom massarray compact system
( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by <t>MassARRAY</t> shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).
Massarray Compact System, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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agena massarray® platform  (CapitalBio Corporation)
90
CapitalBio Corporation agena massarray® platform
( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by <t>MassARRAY</t> shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).
Agena Massarray® Platform, supplied by CapitalBio Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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massarray epityper platform  (Sequenom)
90
Sequenom massarray epityper platform
( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by <t>MassARRAY</t> shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).
Massarray Epityper Platform, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by MassARRAY shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).

Journal: Scientific Reports

Article Title: Prenatal maternal stress and wheeze in children: novel insights into epigenetic regulation

doi: 10.1038/srep28616

Figure Lengend Snippet: ( A ) Based on the histone modification data the DMR (WGBS: Δmeth = 28%) identified in the NMUR1 gene is located in an enhancer region (ENCODE/Active Elements (own ChIP-Seq data)). ( B ) Validation of the NMUR1 DMR (7 CpGs, chr2: 232,394,701–232,394,805) in the total cohort by MassARRAY shows that an increase of the maternal stress level leads to a significant elevation in the methylation level in children at time of birth. In highly stressed children this methylation increase corresponds to a significantly decreased mRNA expression of NMUR1 (mean +/− 95% CI, whiskers +/− non-outlier range). Relationship between NMUR1 methylation and maternal stress score were determined by multiple regression analysis adjusted for gender of the child, birth week, age of the mother, mode of delivery, maternal smoking/-medication during pregnancy, parental history of atopy and cell composition. ( C ) Relationship of interleukin-4, -5 and -6 secretion at time of birth and NMUR1 methylation. Data are presented as ratios of the mean (MR) and 95% confidence intervals. Models were adjusted for known confounders of interleukin concentrations in cord blood (month of birth, mode of delivery, gender of the child, parental history of atopy, smoking during pregnancy and cell composition).

Article Snippet: Methylation differences of selected differentially methylated regions (DMRs) were assessed using the MassARRAY system (Sequenom Inc./Agena Bioscience GmbH, Hamburg Germany), a mass spectrometry based approach for targeted methylation analysis .

Techniques: Modification, ChIP-sequencing, Biomarker Discovery, Methylation, Expressing